Thermus thermophilus muts
WebbThermus thermophilus MutS, a thermostable mismatch-recognizing protein, is utilized in PCR to suppress nonspecific amplification by preventing synthesis from mismatched primers. T. thermophilus RecA also decreases nonspecific amplification by promoting proper hybridization between the primer and template. Webb21 mars 2013 · In 1995 and 1996, Wetmur issued two important US patents (5,877,280 and 6,294,325) [12,13] for the use of MutS proteins from hyperthermophiles, including …
Thermus thermophilus muts
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WebbCiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): The mutS gene, implicated in DNA mismatch repair, was cloned from an extremely thermophilic bacterium, Thermus thermophilus HB8. Its nucleotide sequence encoded a 819-amino acid protein with a molecular mass of 91.4 kDa. Its predicted amino acid sequence showed … WebbDescription. DNA Mismatch Repair Protein MutS Thermus Aquaticus Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 829 amino …
Webb1 mars 2003 · The mutS gene from the thermophilic bacterium Thermus thermophilus was PCR amplified, cloned, and expressed in Escherichia coli. The recombinant MutS … Webb1 mars 2011 · MutS is a key enzyme in DNA mismatch repair (MMR) that corrects mismatched bases produced during DNA replication and other biological processes [ 1, …
WebbAbstract. Bacteria of the genus Thermus have been isolated from many natural and artificial thermal environments throughout the world. The first strains of the type species Thermus aquaticus were isolated from neutral and alkaline hot springs in Yellowstone National Park, USA (Brock and Freeze, 1969). Since then, strains have also been isolated ... Webb1 apr. 2003 · Thermus thermophilus MutS protein hydrolyzed ATP to ADP and Pi, and its activity was maximal at 80°C. The kinetic parameters of the ATPase activity at 65°C …
Webb1 feb. 1996 · Thermus thermophilus MutS protein bound specifically with G-T mismatched DNA even at 60 degrees C. Free full text . Nucleic Acids Res. 1996 Feb 15; 24(4): …
WebbDNA mismatch repair (MMR) corrects mismatched base pairs mainly caused by DNA replication errors. The fundamental mechanisms and proteins involved in the early reactions of MMR are highly conserved in almost all organisms ranging from bacteria to human. The significance of this repair system is also indicated by the fact that defects in … humana preferred providers listWebbFukui K, Masui R, Kuramitsu S (2004) Thermus thermophilus MutS2, a MutS paralogue, possesses an endonuclease activity effected by MutL J Biochem 135, 375-384. Goto M, … humana preferred provider chiropracticWebbNo. 48 Thermus thermophilus HB8 の DNA ミスマッチ修復システム 福井健二1，飯野均1,2，満足美穂1,2，中川紀子1,2，増井良治1,2，倉光成紀1,2 Kenji fukui1, Hitoshi Iino1,2 Miho Manzoku1 Noriko Nakagawa1,2 Ryoji Masui1,2 and Seiki Kuramitsu1,2 （1 理研・播磨研，2 大阪大学・院理） (1RIKEN/Harima Inst., 2Osaka Univ. Grad. Sch. Sci.) humana premier open access provider listWebbttMutL, Thermus thermophilus MutL; ttMutS, Thermus thermophilus MutS. FEBS Journal 280 (2013) 3467–3479 ª 2013 FEBS 3467. change, resulting in the formation of a clamp … holism and reductionism simply psychologyWebb28 apr. 2011 · Oxidative stress generates harmful reactive oxygen species (ROS) that attack biomolecules including DNA. In living cells, there are several mechanisms for … humana preferred prescription listWebb高度好熱菌Thermus thermophilus HB8 をモデル生物とした 「丸ごと一匹プロジェクト」の進捗状況 Progress in the Whole Cell Project of a Model Organism, Thermus … humana preferred rx plan phone numberWebb22 mars 2010 · Furthermore, we have determined the structure of the entire complex I from Thermus thermophilus at 4.5 A resolution. The L-shaped assembly consists of the alpha-helical model for the membrane domain, with 63 TM helices, and the known structure of the hydrophilic domain. The architecture of the complex provides strong clues about the … holism anthropology definition quizlet